African Health OER Network: Xanthoproteic's Test - Colour Reaction of Proteins

Logo of Kwame Nkrumah University of Science and Technology
Term:
2012
Published:
November 30, 2012
Revised:
June 9, 2015

Xanthoprotein is a yellow acid substance formed by the action of hot nitric acid on albuminous or protein matter and is changed to a deep orange-yellow colour by the addition of ammonia. The colour reaction is used to identify such proteins in the Xanthoproteic Test.

Primary Author: Dr. George Koffuor

Student Volunteer: Manasseh Amenyedokui

Multimedia Support: Benjamin Prempeh, John Kofi Dogbey, Ella Kasanga

Institution: Kwame Nkrumah University of Science and Technology

Learning Objectives

  • Write a well-balanced equation reaction for Xanthoprotiec test
  • Distinguish between amino-acids with a phenyl ring, a phenol or an indole group from other amino acids
  • Describe the test and positive result.
Logo of Kwame Nkrumah University of Science and Technology
Term:
2012
Published:
November 30, 2012
Revised:
June 9, 2015

Demos

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Xanthoproteic's Test - Colour Reaction of Proteins

George Koffuor